出版社：Defence Scientific Information & Documentation Centre
摘要：The mouse monoclonal and polyclonal antibodies were produced for the detection of intracellular pathogenand potential warfare agent Francisella tularensis. Antibody titers obtained were 1:640 for polyclonal antibodiesand 1:320 for monoclonal antibodies. Both antibodies were used in the indirect enzyme-linked immunosorbentassay (ELISA) found to detect F. tularensis whole cells. The limit of detection was 5.4×106 CFU/ml for polyclonalantibodies and 6.9×106 CFU/ml for monoclonal antibodies. The value sample could be distinguished from anyconcentration of another gram-negative bacterium: Escherichia coli. Defence Science Journal, 2008, 58(5), pp.698 -702 , DOI:http://dx.doi.org/10.14429/dsj.58.1693
