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  • 标题:Flow-cytometric Analysis of Bacillus anthracis Spores
  • 本地全文:下载
  • 作者:D. V. Kamboj ; G. S. Agarwal ; B. S. Dwarakanath
  • 期刊名称:Defence Science Journal
  • 印刷版ISSN:0976-464X
  • 出版年度:2006
  • 卷号:56
  • 期号:5
  • 页码:769-774
  • DOI:10.14429/dsj.56.1940
  • 语种:English
  • 出版社:Defence Scientific Information & Documentation Centre
  • 摘要:Flow-cytometric technique has been established as a powerful tool for detection and identification of microbiological agents. Unambiguous and rapid detection of Bacillus anthracis spores has been reported using immunoglobulin G-fluorescein isothiocyanate conjugate against live spores. In addition to the high sensitivity, the present technique could differentiate between spores of closely related species, eg, Bacillus cereus and Bacillus subtilis using fluorescence intensity. The technique can be used for detection of live as well as inactivated spores making it more congenial for screening of suspected samples of bioterrorism.
  • 关键词:Flow cytometry, Bacillus anthracis, bacterial spores, FITC, immunofluorescence, bioterrorism, fluorescein isothiocyanate-labelled antibodies, fluorescein isothiocyanate
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