摘要：This study was undertaken to investigate whether treatment with gentamicin plus clindamycin administered subcutaneously could prevent or eradicate translocation of Enterococcus faecalis from the gastrointestinal (GI) tract of mice. The susceptibility of mice to enteric colonisation with an exogenous, streptomycin-resistant strain of E. faecalis was found to be greatly increased when the animals were fed 5 mg of streptomycin sulphate per millilitre in their drinking water. After orogastric challenge with approximately 106 viable organisms, the small intestine, caecum and large intestine of 100 per cent of the streptomycin-treated animals, but of none of the control animals, were colonised with enterococci. Similarly, translocation of E. faecalis to liver, spleen and heart occurred in all of the streptomycin-treated animals, but in none of the untreated controls. Subcutaneous administration of gentamicin plus clindamycin to streptomycin-treated mice concomitant with orogastric enterococcal challenge significantly reduced intestinal populations of these organisms, but not sufficiently to prevent their translocation to extraintestinal sites. When treatment with gentamicin plus clindamycin was delayed for 1 wk following orogastric challenge with E. faecalis, intestinal population sizes of the enterococcus increased significantly, yet the percentage of animals demonstrating translocation of the organism decreased. We conclude that treatment with gentamicin plus clindamycin is ineffective in either preventing enterococcal translocation or reducing established populations of E. faecalis in this experimental model.Keywords: Translocation; Enterococcus; Clindamycin; Bacterial resistance.
