摘要：Intestinal suspensions containing segmented filamentous bacteria (SFBs) were given orally to germ-free mice in an attempt to pave the way for the production of mice mono-associated with SFBs. The effects of dilution, sonication and chloroform/ethanol treatment of intestinal suspensions on SFB colonisation were investigated. Colonisation density of SFBs in the germ-free mice was dependent on the number of SFBs in the administered suspension. Sonication of suspensions had no effect. Chloroform/ethanol treatment of suspensions resulted in the production of di-associated mice, containing both SFBs and a non-characterised clostridium in their small intestine. It is suggested that, through specific elimination of the clostridium, these di-associated animals may be used to produce mice containing SFBs alone.Keywords: Colonisation; Intestinal bacteria; Mouse; Segmented filamentous bacteria.
