摘要:A new selective and differentiative medium for the isolation of Bacteroides fragilis is described. It consists of a Brain Heart Infusion Agar base supplemented with yeast extract, cysteine hydrochloride, haemin, menadione, normal human plasma, bromothymol blue, vancomycin, amikacin, and glucose. If amikacin—resistant aerobic gram—negative bacteria are present, they are colour differentiated due to the presence and metabolism of glucose (0.1%, w/v). Bacteroides fragilis was the only bacterium to form yellow colonies on this medium, whereas a plethora of other aerobic and anaerobic species studied, formed blue colonies. This medium, Bacteroides Amikacin Vancomycin Agar (BAVA) could be useful in Clinical Microbiology laboratories.Keywords: Bacteroides fragilis, selective medium, differentiative medium.