摘要：Objective: An emerging issue of probiotic products is the antibiotic resistance of the strains used. The aim of this study was to determine the susceptibility of the isolates of 10 probiotic products available in Italy. Materials and methods: The susceptibility of 15 strains of Lactobacillus spp., 5 Streptococcus salivarius ssp. thermophilus, 1 Enterococcus faecium and 8 Bifidobacterium spp. to several groups of antibacterial agents was determined by E-test using MRS agar for Lactobacillus spp. and E. faecium and MH agar+5% sheep blood for S. thermophilus, with different conditions of incubation. For Lactobacillus, S. thermophilus and E. faecium the MICs obtained by E-test were compared to the MICs by broth microdilution test obtained following CLSI M45-A (2006) and CLSI M100-S17 (2007) guidelines. Results: The broth microdilution test resulted in MICs identical to those obtained with the E-test or in MICs with differences of 1 or 2 log dilution steps. All the strains of Lactobacillus were susceptible to ampicillin. Species-dependent antibiotic susceptibility was detected for cephalosporins; gentamicin and ciprofloxacin had variable activity. Intrinsic resistance to vancomycin was confirmed for L. paracasei, L. salivarius and L. plantarum. Atypical resistance to erythromycin was detected in one strain of L. salivarius. The strains of Bifidobacterium were susceptible to ampicillin, cefotaxime and erythromycin. The strains of E. faecium were susceptible to the tested antibiotics; the strain of S. thermophilus was resistant only to ciprofloxacin. The observed resistance in the strains used in the Italian probiotic products tested seemed to be intrinsic except for erythromycin in one L. salivarius strain.Key words: antibiotic susceptibility, probiotic, lactic acid bacteria, bifidobacteria
