摘要：Previous studies describing the inactivation of toxins A and B of Clostridium difficile by trypsin and chymotrypsin used impure toxin and protease preparations. We repeated these studies using highly purified toxin, and trypsin and chymotrypsin that were not contaminated with each other. Our findings show that toxin A is resistant to trypsin but not chymotrypsin and that toxin B is inactivated by both proteases. Neither toxin was activated by the proteases.Keywords: C. difficile; Toxin A; Toxin B; Proteases.
