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  • 标题:Detection of Fusobacterium Species in Human Feces Using Genus-Specific PCR Primers and Denaturing Gradient Gel Electrophoresis
  • 本地全文:下载
  • 作者:Jens Walter ; Dirk Margosch ; Walter P. Hammes
  • 期刊名称:Microbial Ecology in Health and Disease
  • 印刷版ISSN:1651-2235
  • 出版年度:2002
  • 卷号:14
  • 期号:3
  • DOI:10.3402/mehd.v14i3.8240
  • 语种:English
  • 出版社:Microbial Ecology in Health and Disease
  • 摘要:Denaturing gradient gel electrophoresis of 16S rDNA fragments generated with a genus-specific PCR system was used to detect Fusobacterium species in human feces, with the exception of F. prausnitzii. Out of 30 fecal samples, four contained fusobacteria at numbers close to the detection-limit of approximately 105 cells per g, thus questioning a substantial role for these species in the intestinal flora.
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