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  • 标题:CHARACTERIZATION OF XYLANASE FROM A XYLANOLYTIC- THERMOPHILIC BACTERIUM ISOLATED FROM GUNUNG PANCAR HOT SPRING, WEST JAVA
  • 本地全文:下载
  • 作者:ANTONIUS SUWANTO ; DWI SURYANTO ; ANJA MERYANDINI
  • 期刊名称:BIOTROPIA
  • 印刷版ISSN:1907-770X
  • 出版年度:2001
  • 期号:17
  • DOI:10.11598/btb.2001.0.17.167
  • 语种:English
  • 出版社:SEAMEO BIOTROP
  • 摘要:A xylanolytic-thermophilic bacterium (IT-08) was isolated from Gunung Pancar Hot Spring after two days of enrichment in Modified Thermus Medium (MTM) supplemented with 0.5% oat spelt xylan. 16S- rRNA sequence analysis indicated that IT-08 resembles Bacillus thermoleovorans, a species of thermophilic bacteria. When grown on xylan containing media, IT-08 produces a thermoactive xylanase. Xylanase from IT- 08 was active at temperatures between 40 and 100°C, at pH values between 4.0 and 9.0 with optimum values obtained at 80°C and pH 6.0, respectively. SDS-PAGE and zymogram analysis demonstrated that a crude xylanase complex of IT-08 comprised two active bands with molecular masses of 78 and 60 kDa. Keywords: xylanase / xylanolytic-thermophilic bacteria
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