首页    期刊浏览 2024年12月03日 星期二
登录注册

文章基本信息

  • 标题:Enhanced Production Level of Quinine in Cell Suspension Culture of Cinchona ledgeriana Moens by Paclobutrazol
  • 本地全文:下载
  • 作者:Diah Ratnadewi
  • 期刊名称:BIOTROPIA
  • 印刷版ISSN:1907-770X
  • 出版年度:2013
  • 卷号:20
  • 期号:1
  • DOI:10.11598/btb.2013.20.1.291
  • 语种:English
  • 出版社:SEAMEO BIOTROP
  • 摘要:Quinine is one of the major alkaloids in Cinchona spp . , and it is used both as a medication and as a drink additive. The plant produces most of its alkaloids in the bark after 6–8 years of age . R epeated harvests can be performed until the plant dies , but only after every 3–5 years. We tested an improved method for culturing cell suspension s of Cinchona ledgeriana t o investigate the possibility of increasing production of quinine. The clone QRC 315 was treated with either a growth retardant ( abscisic acid or paclobutrazol ) or precursor feeding of L-tryptophan. To generate stress, we applied m annitol at 5 . 3 g/L combined with sucrose at a lower concentration (20 g/L) , and we used sucrose at 30 g/L as the control. Paclobutrazol ( 7 mg/L ) significantly suppressed cell growth and produced the highest level of quinine (11%) after 7 weeks of culture. L-tryptophan also reduced cell growth , but without any positive effect in the production of quinoline. The highest amount of quinine per culture flask, however, resulted in cells treated with 3 mg/L abscisic acid. Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;}
国家哲学社会科学文献中心版权所有